Transcriptome analysis provides insights into a molecular mechanism of histamine response in the cyprid larvae of Amphibalanus amphitrite

Barnacles are notorious marine fouling creatures; their planktonic cyprid larvae attach to material substrates and metamorphose. Histamine has shown great importance in regulating settlement metamorphosis. This study aimed investigate the mechanisms of histamine-induced larval settlement. Cyprids were exposed histamine or loratadine, an anti-histamine compound. The percent settlements histamine- anti-histamine-treated cyprids significantly higher lower, respectively, than control group. Transcriptomic analyses showed that histamine-treated had 18498 differentially expressed genes (DEGs, 14531 up-regulated, 3967 down-regulated) group 18055 DEGs (17237 818 comparison untreated controls. In both treatment groups, significant enrichment involved mitogen-activated protein kinase signaling pathway was observed. Based on results bioassays, we set 4 filter conditions perform DEG analyses, 19 selected as functional related categories these included structural proteins, spider silk energy metabolism cement glycosyl multifunctional proteins. AdipoR up-regulated but down-regulated activity adenosine monophosphate-activated kinase, a downstream AdipoR, increased decreased Our provide new insights into molecular underlying barnacle identify important gene can affect cyprids, likely through metabolism.